Author : Na Sun … [et al.].

Source : Journal of Food Science. 84(5)2019:1002-1011

Abstract : Although phosphatidylethanolamine (PE) is an important functional phospholipid, there have been very few reports on its antioxidant activity and corresponding molecular composition. Crude PE was extracted from egg yolk with various solvents, and response surface modeling was carried out to determine the optimum extraction conditions for PE, under which the PE content in extracts reached 58.94 µg/mL. The crude PE was purified using silica gel‐based column chromatography. High‐purity PE (98%), identified by high‐performance liquid chromatography‐evaporative light‐scattering detector, was obtained using isocratic elution with a mixed solvent (chloroform: methanol: acetic acid = 18:5:1) eluent. PE purified from egg yolk exhibited high radical‐scavenging activity, determined by electron paramagnetic resonance (EPR). The result was attributed to the high unsaturated fatty acids (83.10%) content in egg yolk PE, and the unsaturated fatty acids were identified as PE‐16:0/18:1Δ9, PE‐16:0/18:2Δ9,12, PE‐16:0/20:4Δ5,8,11,14, PE‐18:0/18:1Δ9, PE‐18:0/18:2Δ9,12, and PE‐18:0/20:4Δ5,8,11,14 by MALDI‐TOF MS combined with gas chromatography mass spectrometry.

Subject : Antioxidant activity. Egg yolk. Molecular composition. Phosphatidylethanolamine. Purification.